Journal
CHEMICAL COMMUNICATIONS
Volume 47, Issue 9, Pages 2571-2573Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c0cc04292g
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The two opposing yeast copper regulators, Ace1 and Mac1, were converted into two fluorescent probes, Ace1-FRET and Mac1-FRET, which selectively and sensitively respond to Cu+. The Cu+-binding curves obtained for both regulators as well as for the copper storage proteins Cup1 and Crs5 show that free copper(I) is maintained in a narrow window inside yeast.
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