4.7 Article

A V-shaped cationic dye for nonlinear optical bioimaging

Journal

CHEMICAL COMMUNICATIONS
Volume 47, Issue 26, Pages 7374-7376

Publisher

ROYAL SOC CHEMISTRY
DOI: 10.1039/c1cc12163d

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Funding

  1. Spanish Ministerio de Ciencia e Innovacion [CTQ2008-04313/BQU]
  2. Portuguese Fundacao para a Ciencia e a Tecnologia [PTDC/CTM-POL/114367/2009]
  3. Universidad de Alcala

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A symmetric cationic molecule with D-pi-A(+)-pi-D architecture was synthesized with high two-photon absorption cross-section (sigma(2) approximate to 1140 GM). Application as a marker in fluorescence microscopy of living cells revealed its presence inside the cell staining vesicular shape organelles in the cytoplasm. Fluorescence lifetime imaging microscopy shows that it is also able to penetrate within the nucleus.

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