Journal
CHEMICAL COMMUNICATIONS
Volume 47, Issue 15, Pages 4502-4504Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c1cc00024a
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Funding
- National Key Basic Research Foundation of China [2010CB912300]
- National Natural Science Foundation of China [91013005]
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A concise route was developed for the facile synthesis of a cyclic pyrrolysine analogue bearing an azide handle. Directed evolution enabled the encoding of this non-natural amino acid in both prokaryotic and eukaryotic cells, which offers a highly efficient approach for the site-specific protein labeling using click chemistry.
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