Journal
INTERNATIONAL JOURNAL OF ANDROLOGY
Volume 23, Issue -, Pages 89-91Publisher
BLACKWELL SCIENCE LTD
DOI: 10.1046/j.1365-2605.2000.00025.x
Keywords
cryptorchid; integrin; spermatogonial stem cell; steel
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Funding
- EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH &HUMAN DEVELOPMENT [R01HD036504] Funding Source: NIH RePORTER
- NICHD NIH HHS [HD 36504] Funding Source: Medline
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Spermatogenesis is a complex, highly organized process originated from stem cell spermatogonia. Because there are very few stem cells and they can only be defined by their function, the identification and isolation of these cells has been very difficult. By using a spermatogonial transplantation assay system, we have identified alpha(6)- and beta(1)-integrin expression on stem cells, and cells isolated with these antigens were significantly enriched in stem cells. This is the first demonstration of spermatogonial stem cell-associated antigens. Analysis of two infertile mouse models, Steel/Steel(Dickie) (Sl/Sl(d)) and experimental cryptorchidism, showed that the number of stem cells is reduced in Sl/Sl(d) testis. Whereas cryptorchid testes are greatly enriched for stem cells, and one in 200 cells is a stem cell. These techniques will provide an important starting point for further purification and characterization of spermatogonial stem cells.
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