Assembly of F0F1-ATPase into solid state nanoporous membrane

A novel ATPase/nanoporous membrane system was prepared. In this system, the activity of F0F1-ATPase was preserved. The two sides of F0F1-ATPase were successfully separated macroscopically, and the chemical environments of the two sides could be manipulated in situ individually and freely. Furthermore, this system was also provided with mobility and reusage.