4.7 Article

Development of a system to sensitively and specifically visualize c-fos mRNA in living cells using bispyrene-modified RNA probes

Journal

CHEMICAL COMMUNICATIONS
Volume 47, Issue 14, Pages 4204-4206

Publisher

ROYAL SOC CHEMISTRY
DOI: 10.1039/c0cc04639f

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Funding

  1. Japan Society for the Promotion of Science (JSPS) [21350093]
  2. Grants-in-Aid for Scientific Research [22750154] Funding Source: KAKEN

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In situ visualization of c-fos mRNA was shown both in fixed cells and in living cells using bispyrene-modified 2'-O-methyl RNA probes (OMUpy2) or phosphorothioate modified OMUpy2 (OMUpy2-S), which was RNA-specific with high sensitivity, and a system for time-lapse imaging of c-fos mRNA was successfully developed.

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