Journal
CANADIAN JOURNAL OF PLANT PATHOLOGY-REVUE CANADIENNE DE PHYTOPATHOLOGIE
Volume 22, Issue 3, Pages 258-264Publisher
CANADIAN PHYTOPATHOL SOC
DOI: 10.1080/07060660009500473
Keywords
Hordeum vulgare; Pyrenophora teres; Drechslera teres; random amplified polymorphic DNA; markers; genetic structure
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The genetic structure in two Swedish populations of Pyrenophora teres, causal agent of net blotch of barley, is described. Each of the two populations consisted of 64 monoconidial isolates, hierarchically sampled in a farmers' field of cv. Golf and a field of cv. Svani. The two fields were located in western Sweden. 20 km apart. Isolates were obtained from infected barley leaves sampled at eight sites in each held. Three random amplified polymorphic DNA (RAPD) primers, with a total of 19 reproducible loci, were used from the initial screening of 70 RAPD primers. The variation for the RAPD markers was large in both populations, resulting in mean gene diversities of 0.182 and 0.216, respectively. The total gene diversity was 0.236 and 0.253, respectively, The differentiation among sampling sites was relatively high with coefficient of gene differentiation values of 0.219 and 0.180 for the two fields, but only 5.3% of the variation was due to differentiation between the two populations. A phenogram based on genetic distances among sites also illustrates that the variation is distributed on a small scale. No clear differentiation between fields or among sites was revealed. A very low level of gametic disequilibrium was found. A raised level of disequilibrium in the P. teres cv. Svani population indicated an effect of asexual reproduction. The overall pattern of the genomic structure is that of a random mating sexual species.
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