Journal
BIOTECHNIC & HISTOCHEMISTRY
Volume 75, Issue 2, Pages 79-84Publisher
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.3109/10520290009064151
Keywords
alcian blue; alizarin red; bone; cartilage; Diplodus sp.; Halobatrachus didactilus; larvae; skeleton; Solea senegalensis; Sparus aurata
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We have developed a procedure for staining cartilage and bone in fish larvae as small as 2 mm (notochord length), for which standard alcian blue/alizarin red procedures did not give positive and/or consistent results, Small calcified structures only 100-200 mu m in length can be clearly visualized. The method is suitable for both ontogenic studies during early stages of skeletal development in most marine fishes (e.g., Sparus aurata L,, Solea senegalensis Kaup), whose larvae at hatching are often only a few millimeters long and for detecting skeletal abnormalities In small larvae, This procedure can also be used for specimens that have been preserved in 100% ethanol for up to two years.
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