Journal
BIOTECHNIC & HISTOCHEMISTRY
Volume 75, Issue 2, Pages 54-78Publisher
TAYLOR & FRANCIS LTD
DOI: 10.3109/10520290009064150
Keywords
cytogenetics; fluorescence in situ hybridization
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Fluorescence in situ hybridization (FISH) is a powerful technique for detecting DNA or RNA sequences in cells, tissues and tumors. This molecular cytogenetic technique enables the localization of specific DNA sequences within interphase chromatin and metaphase chromosomes and the identification of both structural and numerical chromosome changes. FISH is quickly becoming one of the most extensively used cytochemical staining techniques owing to its sensitivity and versatility, and with the improvement of current technology and cost effectiveness, its use will surely continue to expand. Here we review the wide variety of current applications and future prospects of FISH technology.
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