4.7 Article

Separation of proteins using supramolecular gel electrophoresis

Journal

CHEMICAL COMMUNICATIONS
Volume 47, Issue 37, Pages 10344-10346

Publisher

ROYAL SOC CHEMISTRY
DOI: 10.1039/c1cc13826j

Keywords

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Funding

  1. Daicel Chemical Industry Award in Synthetic Organic Chemistry, Japan [2206]
  2. Grants-in-Aid for Scientific Research [23590088] Funding Source: KAKEN

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An amphiphilic low-molecular-weight hydrogelator 1 was synthesized. A tris-glycine-SDS solution gel of 1 was applied for electrophoresis to separate proteins. Centrifugation of a mixture of protein and a hydrogel of 1 enabled the recovery of protein. Various combinations of proteins were applied for supramolecular gel electrophoresis (SUGE), and remarkably poor mobility for small proteins (<45 kDa) was found.

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