4.7 Article

Fluorescence detection for DNA using hybridization chain reaction with enzyme-amplification

Journal

CHEMICAL COMMUNICATIONS
Volume 46, Issue 18, Pages 3089-3091

Publisher

ROYAL SOC CHEMISTRY
DOI: 10.1039/c000166j

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Funding

  1. National Natural Science Foundation of China [20775038]
  2. Excellent Young Scientists Foundation of Shandong Province [JQ200805]
  3. University Doctoral Foundation of the Ministry of Education [200804260001]

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A signal amplification based on a combination of hybridization chain reaction (HCR) and enzyme-enhancement was applied in the study of a novel biosensor. The target sequence was added as catalyst which was simply transmitted but did not disappear as the DNA double strand grew on the surface of magnetic beads (MBs).

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