Journal
PLANT MOLECULAR BIOLOGY REPORTER
Volume 18, Issue 2, Pages 109-115Publisher
SPRINGER
DOI: 10.1007/BF02824018
Keywords
banana; fruit tissue; Musa acuminata; polysaccharides; RNA extraction
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Isolation of high quality RNA from ripening banana fruit tissue is difficult due to high levels of polysaccharides and other substances that interfere when using conventional procedures for RNA isolation. These substances not only decrease the yield but the quality of RNA is almost unusable. We describe here a simple RNA procedure that effectively removes these contaminating substances without affecting the yield. Following this procedure, we routinely obtained 80-150 mu g of total RNA per g fresh tissue. The RNA is of good quality and suitable for northern analysis, RT-PCR and cDNA library construction.
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