Journal
CHEMICAL COMMUNICATIONS
Volume 46, Issue 43, Pages 8195-8197Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c0cc02864a
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Funding
- NIH
- European Commission
- BBSRC
- Oxford Nanopore Technologies
- NATIONAL HUMAN GENOME RESEARCH INSTITUTE [R01HG003709] Funding Source: NIH RePORTER
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Two DNA bases, 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (hmC), marks of epigenetic modification, are recognized in immobilized DNA strands and distinguished from G, A, T and C by nanopore current recording. Therefore, if further aspects of nanopore sequencing can be addressed, the approach will provide a means to locate epigenetic modifications in unamplified genomic DNA.
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