Journal
CHEMICAL COMMUNICATIONS
Volume 46, Issue 47, Pages 9007-9009Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c0cc02683b
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Funding
- MEC [MAT2008-03079/NAN, CSD2006-00012]
- Juan de la Cierva scholarship
- ICREA Funding Source: Custom
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A novel nanoparticle-based enhanced methodology for the detection of ssDNA using nanoporous alumina filter membranes, containing pores of 200 nm in diameter, is reported. The blockage of the pores due to the hybridization is detected by measuring the decrease in the differential pulse voltammetric response of the [Fe(CN)(6)](4-/3-) redox indicator and using screenprinted carbon electrodes as transducing platform. Furthermore, 20 nm gold nanoparticle (AuNPs) tags are used in order to increase the sensitivity of the assay. The enhancement mechanism of DNA detection is due to an additional blocking effect induced by hybridization reaction by bringing AuNPs inside the pores. The developed methodology can be extended to other biosensing systems with interest not only for DNA but also for proteins and cells. The developed nanochannel/nanoparticle biosensing system would have enormous potential in future miniaturized designs adapted to mass production technologies such as screen-printing technology.
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