4.7 Article

A microfluidic approach for high-throughput droplet interface bilayer (DIB) formation

Journal

CHEMICAL COMMUNICATIONS
Volume 46, Issue 10, Pages 1620-1622

Publisher

ROYAL SOC CHEMISTRY
DOI: 10.1039/b924897h

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Funding

  1. EPSRC [EP/G00465X/1]
  2. RCUK [EP/D048664/1]
  3. GSK
  4. EPSRC [EP/D048664/1] Funding Source: UKRI
  5. Engineering and Physical Sciences Research Council [EP/D048664/1, EP/G00465X/1] Funding Source: researchfish

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We present a simple, automated method for high-throughput formation of droplet interface bilayers (DIBs) in a microfluidic device. We can form complex DIB networks that are able to fill predefined three dimensional architectures. Moreover, we demonstrate the flexibility of the system by using a variety of lipids including 1,2-diphytanoyl-sn-glycero-3-phosphocholine ( DPhPC) and 1,2-dioleoyl-sn-glycero-3-phosphocholine ( DOPC).

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