4.7 Article

Electrochemical DNA sandwich assay with a lipase label for attomole detection of DNA

Journal

CHEMICAL COMMUNICATIONS
Volume 46, Issue 11, Pages 1836-1838

Publisher

ROYAL SOC CHEMISTRY
DOI: 10.1039/b924627d

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Funding

  1. Danish Food Industry Agency (DFFE)
  2. Danish National Research Foundation
  3. Carlsberg Foundation

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A fast and sensitive electrochemical lipase-based sandwich hybridization assay for detection of attomole levels of DNA has been developed. A combination of magnetic beads, used for pre-concentration and bioseparation of the analyte with a lipase catalyst label allowed detection of DNA with a limit of 20 amol.

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