4.7 Article

Enzyme-assisted binary probe for sensitive detection of RNA and DNA

Journal

CHEMICAL COMMUNICATIONS
Volume 46, Issue 46, Pages 8761-8763

Publisher

ROYAL SOC CHEMISTRY
DOI: 10.1039/c0cc03248d

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Funding

  1. UCF Office of Research and Commercialization, College of Sciences, Chemistry Department
  2. NHGRI [R21 HG004060]
  3. NATIONAL HUMAN GENOME RESEARCH INSTITUTE [R21HG004060] Funding Source: NIH RePORTER

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The new enzyme-assisted assay for DNA/RNA detection provides real-time fluorescent signal readout along with low limit of detection and high discrimination power toward a single-base substitution. Requiring only two new unmodified DNA oligonucleotides for the detection of each new analyte, the assay is an efficient tool for low-cost analysis of multiple analytes.

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