Journal
CHEMICAL COMMUNICATIONS
Volume 46, Issue 43, Pages 8154-8156Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c0cc01968b
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Funding
- U.S. National Institutes of Health [GM068122]
- NSF [CHE-0639053]
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM068122] Funding Source: NIH RePORTER
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Fluorescence-quenched nucleic acid probes with reactive moieties at both the 50 and 30 ends are synthesized and tested for reaction with two adjacent nucleophile-containing DNAs. These probes improve signal to background over singly reactive probes and can discriminate single nucleotide polymorphisms in the target DNA or RNA.
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