Journal
PLANT MOLECULAR BIOLOGY REPORTER
Volume 18, Issue 3, Pages 243-253Publisher
INT SOC PLANT MOLECULAR BIOLOGY
DOI: 10.1007/BF02823995
Keywords
Chinese Spring wheat; chloroplast DNA; clone bank; complete nucleotide sequence; plastome evolution
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Libraries of plasmid clones covering the entire chloroplast (cp) genome of the common wheat, Triticum aestivum cv. Chinese Spring were constructed and assembled into contig-clones. From these, we obtained the complete nucleotide sequence of wheat chloroplast DNA - a 134,540 bp circular DNA (DDBJ accession no. AB042240) containing four species of ribosomal RNA, 30 genes for 20 species of transfer RNA, and 71 protein coding genes. Additionally, we detected five unidentified open reading frames conserved among grasses. Plasmid clones are available on request.
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