4.7 Article

Highly selective detection of Cu2+ utilizing specific binding between Cu-demetallated superoxide dismutase 1 and the Cu2+ ion via surface plasmon resonance spectroscopy

Journal

CHEMICAL COMMUNICATIONS
Volume -, Issue 41, Pages 6171-6173

Publisher

ROYAL SOC CHEMISTRY
DOI: 10.1039/b910666a

Keywords

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Funding

  1. Korea Science & Engineering Foundation [R01-2006-00010239-0]
  2. Ministry of Environment, Korea [101071-028]
  3. WCU (World Class University)
  4. Korea science and Engineering Foundation
  5. Ministry of Education, Science and Technology [400-2008-0230]
  6. Korea Ministry of Science and Technology

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A highly specific interaction between a metal-deficient metalloenzyme and metal ion has been utilized in the selective detection of the metal ion by surface plasmon resonance spectroscopy (SPRS). The use of SPRS and Cu-demetallated superoxide dismutase (E,Zn-SOD1) as a sensing actuator allows one to selectively and in situ detect Cu2+ without any interference that other spectroscopic methods may have.

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