Journal
BIOPHYSICAL JOURNAL
Volume 78, Issue 1, Pages 322-333Publisher
BIOPHYSICAL SOCIETY
DOI: 10.1016/S0006-3495(00)76595-7
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Funding
- NATIONAL HEART, LUNG, AND BLOOD INSTITUTE [R37HL030077, R01HL030077] Funding Source: NIH RePORTER
- NHLBI NIH HHS [HL09412, HL30077] Funding Source: Medline
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We have characterized [Ca](i) decline in voltage-clamped rabbit ventricular myocytes with progressive increases in sarcoplasmic reticulum (SR) calcium load, Backflux through the SR calcium pump is a critical feature which allows realistically small values for SR calcium leak fluxes to be used, Total cytosolic calcium was calculated from the latter part of [Ca](i) decline using rate constants for cellular calcium buffers, Intra-SR calcium buffering characteristics were also deduced, We found that the net SR calcium pump flux and rate of [Ca](i) decline decreased as the SR free [Ca] rose, with pump parameters held constant. We have therefore characterized for the first time in intact myocytes both forward and reverse SR calcium pump kinetics as well as intra-SR calcium buffering and SR calcium leak. We conclude that the reverse flux through the SR calcium pump is an important factor in comprehensive understanding of dynamic SR calcium fluxes.
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