Journal
CHEMICAL COMMUNICATIONS
Volume -, Issue 39, Pages 5880-5882Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/b912025d
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A new method for covalent labeling of a His-tag fused protein with a small reactive probe was developed; this method is based on the complementary interaction between the His-tag and Ni(II)-NTA, which facilitates a nucleophilic reaction between a histidine residue of the tag and the electrophilic tosyl group of the Ni(II)-NTA probe by the proximity effect.
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