Journal
FEBS LETTERS
Volume 480, Issue 2-3, Pages 84-88Publisher
WILEY
DOI: 10.1016/S0014-5793(00)01909-8
Keywords
xanthine oxidase; xanthine dehydrogenase; gene regulation; promoter region; transcription factor; nuclear factor Y
Ask authors/readers for more resources
To study the regulation of the human xanthine oxidoreductase (XOR) gene, we cloned 1.9 kb of the promoter region. In reporter gene assays, a construct encompassing nucleotides between -142 to +42 conferred maximal basal activity of the XOR promoter in 293T cells, in comparison with shorter (-92 to +42) or longer (up to -1937 to +42) constructs. The promoter activity was low in NIH-3T3 cells. The most active construct contained a putative CCAAT motif at -119 to -123, Electrophoretic mobility shift assays showed that this sequence hinds the ubiquitous nuclear factor Y (NF-Y), Mutation of the CCAAT motif (CTGAT) abolished the NF-Y binding and considerably reduced the promoter activity. Our data suggest an important functional role for NF-Y in the transcriptional activation of the human XOR gene. (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B,V, All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available