Journal
FEBS LETTERS
Volume 480, Issue 2-3, Pages 208-212Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0014-5793(00)01938-4
Keywords
carrot cell; cell-free system from plant; demembranated sperm chromatin; nuclear reconstitution; nucleosome
Ask authors/readers for more resources
A cell-free system derived from carrot cell cytosol extract has been developed for reassembling nuclear structure around the added demembranated sperm chromatin of Xenopus. Morphological evidence suggests that reassembled nuclei display the typical characteristics of normal eukaryotic nuclei, such as double-layered nuclear membrane and nuclear pores, Micrococcal nuclease treatment indicates that remodeling of the demembranated sperm chromatin has occurred and the structure of nucleosome is formed during nuclear reconstitution. These data indicate that the nuclear reconstitution can be induced in cell-free systems from plants, and the self-assembly of the nucleus is ubiquitous in both animal and plant cells, (C) 2000 Federation of European Biochemical Societies, Published by Elsevier Science B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available