Journal
JOURNAL OF BACTERIOLOGY
Volume 182, Issue 2, Pages 546-550Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JB.182.2.546-550.2000
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Funding
- NIGMS NIH HHS [GM20509, R01 GM020509] Funding Source: Medline
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM020509] Funding Source: NIH RePORTER
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Previously, we identified a gene (aldA) from Myxococcus xanthus, which we suggested encoded the enzyme alanine dehydrogenase on the basis of similarity to known Aid protein sequences (M, J. Ward, H. Lew, A. Treuner-Lange, and D, R, Zusman, J, Bacteriol. 180:5668-5675, 1998), In this study, we have confirmed that aldA does encode a functional alanine dehydrogenase, since it catalyzes the reversible conversion of alanine to pyruvate and ammonia. Whereas an aldA gene disruption mutation did not significantly influence the rate of growth or spreading on a rich medium, AldA was required for growth on a minimal medium containing L-alanine as the major source of carbon. Under developmental conditions, the aldA mutation caused delayed aggregation in both wild-type (DZ2) and FB (DZF1) strains. Poorly formed aggregates and reduced levels of spores were apparent in the DZ2 aldA mutant, even after prolonged development.
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