Journal
AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY
Volume 182, Issue 1, Pages 60-67Publisher
MOSBY-ELSEVIER
DOI: 10.1016/S0002-9378(00)70491-1
Keywords
acute distention; differentially expressed genes; fetal membranes
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Funding
- EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH &HUMAN DEVELOPMENT [R01HD024314] Funding Source: NIH RePORTER
- NCRR NIH HHS [RR-03061, RR-AI11091RC11] Funding Source: Medline
- NICHD NIH HHS [HD-24314] Funding Source: Medline
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OBJECTIVE: This study was undertaken to identify genes with expression up-regulated by acute distention in the human fetal membranes. STUDY DESIGN: Fetal membrane explants were distended reproducibly in a novel device in vitro for 4 hours, and suppression subtractive hybridization was used to identify the candidate genes for up-regulation of expression in response to this stimulus. The up-regulation in response to distention was confirmed by quantitative Northern blot analysis both after a 4-hour in vitro distention and after labor in vivo. RESULTS: Suppression subtractive hybridization identified 3 genes with expression up-regulated by acute distention: an interferon-stimulated gene encoding a 54-kd protein, the gene for huntingtin-interacting protein 2 (a ubiquitin-conjugating enzyme), and a novel transcript. Expression of each of the distention-responsive genes found to be up-regulated in vitro was also up-regulated in fetal membranes in association with labor. CONCLUSIONS: Suppression subtractive hybridization was successfully applied to a complex tissue, the human fetal membranes, and 3 novel distention-responsive genes were identified. Both acute in vitro distention and labor in vivo up-regulate expression of at least 3 genes in the human fetal membranes.
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