Journal
MOLECULAR AND CELLULAR BIOLOGY
Volume 20, Issue 1, Pages 352-362Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/MCB.20.1.352-362.2000
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Funding
- NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [R37AI039115, P01AI044975, R01AI039115, R01AI042159] Funding Source: NIH RePORTER
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Cryptococcus neoformans is an opportunistic fungal pathogen with a defined sexual cycle. The gene encoding a heterotrimeric G-protein beta subunit, GPB1, was cloned and disrupted. gpb1 mutant strains are sterile, indicating a role for this gene in mating. GPB1 plays an active role in mediating responses to pheromones in early mating steps (conjugation tube formation and cell fusion) and signals via a mitogen-activated protein (MAP) kinase cascade in both MAT alpha and MATa cells. The functions of GPB1 are distinct from those of the G alpha protein GPA1, which functions in a nutrient-sensing cyclic AMP (cAMP) pathway required for mating, virulence factor induction, and virulence. gpb1 mutant strains are also defective in monokaryotic fruiting in response to nitrogen starvation. We show that MATa cells stimulate monokaryotic fruiting of MAT alpha cells, possibly in response to mating pheromone, which may serve to disperse cells and spores to locate mating partners. In summary, the G beta subunit GPB1 and the G alpha subunit GPA1 function in distinct signaling pathways: one (GPB1) senses pheromones and regulates mating and haploid fruiting via a MAP kinase cascade, and the other (GPA1) senses nutrients and regulates mating, virulence factors, and pathogenicity via a cAMP cascade.
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