Journal
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
Volume 381, Issue 1, Pages 25-30Publisher
ACADEMIC PRESS INC
DOI: 10.1006/abbi.2000.1951
Keywords
active site; coulombic interaction; oxometalate; vanadate; vanadium
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Funding
- NIGMS NIH HHS [GM44783] Funding Source: Medline
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM044783] Funding Source: NIH RePORTER
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Pentavalent organo-vanadates have been used extensively to mimic the transition state of phosphoryl group transfer reactions. Here, decavanadate (V10O286-) is shown to be an inhibitor of catalysis by bovine pancreatic ribonuclease A (RNase A). Isothermal titration calorimetry shows that the K-d for the RNase A . decavanadate complex is 1.4 mu M. This value is consistent with kinetic measurements of the inhibition of enzymatic catalysis, The interaction between RNase A and decavanadate has a coulombic component, as the affinity for decavanadate is diminished by NaCl and binding is weaker to variant enzymes in which one (K41A RNase A) or three (K7A/R10A/K66A RNase A) of the cationic residues near the active site have been replaced with alanine. Decavanadate is thus the first oxometalate to be identified as an inhibitor of catalysis by a ribonuclease. Surprisingly, decavanadate binds to RNase A with an affinity similar to that of the pentavalent organo-vanadate, uridine 2',3'-cyclic vanadate. (C) 2000 Academic Press.
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