Journal
NATURE BIOTECHNOLOGY
Volume 18, Issue 9, Pages 959-963Publisher
NATURE AMERICA INC
DOI: 10.1038/79449
Keywords
tissue engineering; mesenchymal stem cells; bone repair; marrow stromal cells; coral
Categories
Ask authors/readers for more resources
Bone lesions above a critical size become scarred rather than regenerated, leading to nonunion. We have attempted to obtain a greater degree of regeneration by using a resorbable scaffold with regeneration-competent cells to recreate an embryonic environment in injured adult tissues, and thus improve clinical outcome, We have used a combination of a coral scaffold with in vitro-expanded marrow stromal cells (MSC) to increase osteogenesis more than that obtained with the scaffold alone or the scaffold plus fresh bone marrow, The efficiency of the various combinations was assessed in a large segmental defect model in sheep, The tissue-engineered artificial bone underwent morphogenesis leading to complete recorticalization and the formation of a medullary canal with mature lamellar cortical bone in the most favorable cases. Clinical union never occurred when the defects were left empty or filled with the scaffold alone, In contrast, clinical union was obtained in three out of seven operated limbs when the defects were filled with the tissue-engineered bone.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available