Journal
NATURE BIOTECHNOLOGY
Volume 18, Issue 3, Pages 309-312Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/73760
Keywords
kinase; capillary zone electrophoresis; peptide; laser-micropipette
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Funding
- NCI NIH HHS [CA09054, R01 CA78858] Funding Source: Medline
- NIAID NIH HHS [KO8AI01513] Funding Source: Medline
- NATIONAL CANCER INSTITUTE [R01CA078858] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [K08AI001513] Funding Source: NIH RePORTER
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We demonstrate a new method for the simultaneous measurement of the activation of key regulatory enzymes within single cells. To illustrate the capabilities of the technique, the activation of protein kinase C (PKC), protein kinase A (PKA), calcium-calmodulin activated kinase II (CamKII), and cdc2 protein kinase (cdc2K) was measured in response to both pharmacological or physiological stimuli. This assay strategy should be applicable to a broad range of intracellular enzymes, including phosphatases, proteases, nucleases, and other kinases.
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