4.3 Article

Structural determination and DPPH radical-scavenging activity of two acylated flavonoid tetraglycosides in oolong tea (Camellia sinensis)

Journal

CHEMICAL & PHARMACEUTICAL BULLETIN
Volume 56, Issue 6, Pages 851-853

Publisher

PHARMACEUTICAL SOC JAPAN
DOI: 10.1248/cpb.56.851

Keywords

Camellia sinensis; oolong tea; quercetin; kaempferol; 1,1-diphenyl-2-pierylhydrazyl

Funding

  1. National Science Council of the Republic of China [NSC 94-2317-B-020-001, NSC 96-2317-B-020-003]

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Two major acylated flavonoid tetraglycosides were isolated from the methanol extract of oolong tea. Their structures were elucidated by spectroscopic methods as quercetin 3-O-[2(G)-(E)-coumaroyl-3G-O-beta-D-glucosyl-3R-O-beta-D-glucosylrutinoside] (1) and kaempferol 3-O-[2(G)-(E)-coumaroyl-3G-O-beta-D-glucosylrutinoside] (2). Compounds 1 and 2 exhibited scavenging activity against DPPH radical with EC50 values of 30.5 and 487.2 mu M, respectively.

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