Journal
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
Volume 375, Issue 1, Pages 55-61Publisher
ACADEMIC PRESS INC
DOI: 10.1006/abbi.1999.1635
Keywords
thyroid; branched-chain amino acids; branched-chain alpha-ketoacid dehydrogenase; branched-chain alpha-ketoacid dehydrogenase kinase; rat; liver; muscle
Categories
Funding
- NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES [R01DK019259] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM051262] Funding Source: NIH RePORTER
- NIDDK NIH HHS [DK 19259] Funding Source: Medline
- NIGMS NIH HHS [R01 GM051262-08, R01 GM051262] Funding Source: Medline
Ask authors/readers for more resources
Hyperthyroidism induced by 3-day treatment of rats with thyroid hormone (T-3; 3,5,3'-triiodothyronine) at 0.1 or 1 mg/kg body wt/day resulted in a reduced activity state (% of enzyme in its active, dephosphorylated state) of the hepatic branched-chain alpha-ketoacid dehydrogenase (BCKDH) complex. One treatment with 0.1 mg T-3/kg body wt caused a significant effect on the activity state of BCKDH complex after 24 h, indicating that the reduction of the activity state was triggered by the first administration of T-3. Hyperthyroidism also caused a stable increase in BCKDH kinase activity, the enzyme responsible for phosphorylation and inactivation of the BCKDH complex, suggesting that T-3 caused inactivation of the BCKDH complex by induction of its kinase, Western blot analysis also revealed increased amounts of BCKDH kinase protein in response to hyperthyroidism, No change in the plasma levels of branched-chain alpha-keto acids was observed in T-3-treated rats, arguing against an involvement of these known regulators of BCKDH kinase activity, Inactivation of the hepatic BCKDH complex as a consequence of overexpression of its kinase may save the essential branched-chain amino acids for protein synthesis during hyperthyroidism (C) 2000 Academic Press.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available