Journal
EXPERIMENTAL HEMATOLOGY
Volume 28, Issue 3, Pages 311-317Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/S0301-472X(99)00151-4
Keywords
mobilization; hematopoietic progenitor cells; anti-VCAM-1 antibody; granulocyte colony-stimulating factor
Categories
Ask authors/readers for more resources
Objective. One of the mechanisms for mobilization of hematopoietic stem cells and progenitor cells is alternation of adhesion molecules, We investigated the mobilization of hematopoietic progenitor cells in blood by administration of anti-vascular cell adhesion molecule (VCAM)-1 antibody (Ab) in mice. Materials and Methods. Twelve- to 14-week-old C57BL/6J mice were injected intravenously with anti-VCAM-1 Ab and anti-very late antigen (VLA)-4 Ab at a dose of 5 mg/kg for 2 days. Results. The number of colony-forming cells (CFCs) in blood was increased 11.4-fold after anti-VCAM-1 Ab treatment, but the number of CFCs was not increased after treatment with anti-VLA-4 Ab. The number of colony-forming unit spleen (CFU-S) also was increased 21.6-fold in the peripheral blood by administration of anti-VCAM-1 Ab, The number of CFCs and CFU-S in the bone marrow of mice treated with anti-VCAM-1 Ab was decreased and that in the spleen also was decreased, On administration of recombinant human granulocyte colony-stimulating factor (125 mu g/kg twice daily) with anti-VCAM-1 Ab, the numbers of CFCs and CFU-S were increased 141.8-fold acid 439-fold, respectively. Conclusion. These observations demonstrated that administration of anti-VCAM-1 Ab induced mobilization of hematopoietic progenitor cells into blood from bone marrow and spleen and that granulocyte colony-stimulating factor has synergistic effects on anti-VCAM-1 Ab-induced mobilization. (C) 2000 International Society for Experimental Hematology, Published by Elsevier Science Inc.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available