Journal
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
Volume 373, Issue 1, Pages 163-174Publisher
ACADEMIC PRESS INC
DOI: 10.1006/abbi.1999.1552
Keywords
estrogen receptor; aryl hydrocarbon receptor; dioxin; antiestrogens; COUP-TF; ERR alpha 1
Categories
Funding
- NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES [R01DK053220] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES [R29ES008088, P20ES006832] Funding Source: NIH RePORTER
- NIDDK NIH HHS [R01 DK 53220] Funding Source: Medline
- NIEHS NIH HHS [1P20 ES06832-12, R29 ES008088, R29 ES08088] Funding Source: Medline
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The molecular mechanisms underlying the apparent cross-talk between estrogen receptor (ER)- and aryl-hydrocarbon receptor (AHR)-mediated activities are unknown. To determine how AHR ligand 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) may inhibit ER action and, conversely, to examine how 17-beta-estradiol (E-2) affects AHR activity, we examined discrete activities of each receptor, i.e., protein-protein interactions, DNA binding, and transcriptional activation. We report that AHR interacts directly with ER alpha, COUP-TF, and ERR alpha 1, in a ligand-specific manner in vitro. Unoccupied or beta-napthoflavone (beta-NF)-occupied AHR showed stronger interaction with ER alpha, COUP-TF, and ERR alpha 1 than when AHR was occupied by the partial antagonist cu-naphthoflavone (alpha-NF), indicating a role for ligand in AHR interaction with these proteins. We also report that AHR interacts with COUP-TF in transfected CV-1 cells. In contrast, the AHR nuclear translocator protein (ARNT) did not interact with COUP-TF, ERR alpha 1, or ER alpha. We next examined the interaction of either ER alpha or COUP-TF with a consensus xenobiotic response element (XRE). Purified ER alpha did not bind the consensus XRE, but COUP-TFI bound the consensus XRE, suggesting a role for COUP-TF as a AHR/ARNT competitor for XRE binding. In transiently transfected MCF-7 human breast cancer cells, overexpression of COUP-TFI inhibited TCDD-activated reporter gene activity from the CYP1A1 promoter. TCDD inhibited estradiol (E-2)-activated reporter gene activity from a consensus ERE and from the EREs in the pS2 and Fos genes, and COUP-TFI did not block the antiestrogenic activity of TCDD. The specific interaction of COUP-TF with XREs and AHR together with the inhibition of TCDD-induced gene expression by COUP-TF suggests that COUP-TF may regulate AHR action both by direct DNA binding competition and through protein-protein interactions. (C) 2000 Academic Press.
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