Journal
NATURE BIOTECHNOLOGY
Volume 18, Issue 1, Pages 91-94Publisher
NATURE AMERICA INC
DOI: 10.1038/72006
Keywords
high-density oligonucleotide probe arrays; fluorescence detection; genotyping; DNA diagnosis
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Funding
- NIGMS NIH HHS [GM48142] Funding Source: Medline
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We have developed a randomly ordered fiber-optic gene array for rapid, parallel detection of unlabeled DNA targets with surface immobilized molecular beacons (MB) that undergo a conformational change accompanied by a fluorescence change in the presence of a complementary DNA target. Microarrays are prepared by randomly distributing MB-functionalized 3-mu m diameter microspheres in an array of wells etched in a 500-mu m diameter optical imaging fiber. Using several MBs, each designed to recognize a different target, we demonstrate the selective detection of genomic cystic fibrosis related targets. Positional registration and fluorescence response monitoring of the microspheres was performed using an optical encoding scheme and an imaging fluorescence microscope system.
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