Journal
GLYCOBIOLOGY
Volume 10, Issue 9, Pages 941-950Publisher
OXFORD UNIV PRESS INC
DOI: 10.1093/glycob/10.9.941
Keywords
fast atom bombardment mass spectrometry; nematode glycosylation; phosphorylcholine containing carbohydrates; structure analysis; Trichinella spiralis
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Funding
- NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [R01AI014490] Funding Source: NIH RePORTER
- NIAID NIH HHS [AI14490] Funding Source: Medline
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Although the presence of phosphorylcholine (PC) in Trichinella spiralis is well established, the precise structure of the PC-bearing molecules is not known. In this paper, we report structural studies of N-glycans released from T.spiralis affinity-purified antigens by peptide N-glycosidase F. Three classes of N-glycan structures were observed: high mannose type structures; those which had been fully trimmed to the trimannosyl core and were sub-stoichiometrically fucosylated; and those with a trimannosyl core, with and without core fucosylation, carrying between one and eight N-acetylhexosamine residues. Of the three classes of glycans, only the last was found to be substituted with detectable levels of phosphorylcholine.
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