Journal
THEORETICAL AND APPLIED GENETICS
Volume 100, Issue 5, Pages 665-669Publisher
SPRINGER
DOI: 10.1007/s001220051337
Keywords
Brassica napus; Sinapis alba; Raphanus sativus; FISH; 5S rDNA genes; 25S rDNA genes
Categories
Ask authors/readers for more resources
Different ribosomal RNA (5S and 25S) genes were investigated simultaneously by fluorescence in situ hybridization (FISH) in Sinapis alba, Raphanus sativus and Brassica napus. The chromosomes of S. alba carried four 5S and six 25S gene sites, and those of R. sativus four sites of each gene, respectively, These two species have one chromosome pair with both rDNA genes; the two are closely located on a short arm of S. alba, while in R, sativus one is distal on the short arm (5S) and the other more proximal on the long arm (25S). In B. napus we have confirmed 12 sites of 25S rDNA. The detection of 5S rDNA genes revealed 14 signals on 12 chromosomes. Of these, six chromosomes had signals for both rDNA genes. The FISH with 5S rDNA probes detected two sites closely adjacent in four chromosomes of B napus. These results are discussed in relation to a probable homoeologous chromosome pair in B. oleracea.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available