Journal
BIOTECHNOLOGY LETTERS
Volume 22, Issue 2, Pages 109-114Publisher
KLUWER ACADEMIC PUBL
DOI: 10.1023/A:1005675920451
Keywords
alcohol oxidase; gene expression; human growth hormone; Pichia pastoris
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Mature human growth hormone (hGH) cDNA was cloned by homologous recombination into the yeast Pichia pastoris genome. The hGH gene expression was placed under the control of the methanol-inducible alcohol oxidase 1 (AOX1) gene promoter and the Saccharomyces cerevisiae alpha-factor signal sequence to direct the secretion of recombinant human growth hormone (rhGH) into the growth medium. O-2-limited induction of recombinant yeast strains in shake tubes with 3 ml of culture medium produced up to 11 mg rhGH l(-1), while high cell density cultures using a 2-l bioreactor produced about 49 mg rhGH l(-1) achieving 40% of total protein of the culture medium supernatant.
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