Journal
GLYCOBIOLOGY
Volume 10, Issue 1, Pages 89-101Publisher
OXFORD UNIV PRESS INC
DOI: 10.1093/glycob/10.1.89
Keywords
CD15; oligosaccharide structural analysis; on-target enzymatic cleavage; Schistosoma mansoni antigenic glycolipids; stage-specific expression
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The oligosaccharide structures of glycolipids from cercariae of the human blood fluke, Schistosoma mansoni, were analyzed in the form of their corresponding, pyridylaminated oligosaccharides by methylation analysis, partial hydrolysis, exoglycosidase treatment, on-target exoglycosidase cleavage and matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry, The six, dominant chemical structures present have been determined as: GalNAc(beta 1-4)Glc1-ceramide; GlcNAc(beta 1-3)GalNAc(beta 1-4)Glc1-ceramide; Gal(beta 1-4)GlcNAc(beta 1-3) GalNAc(beta 1-4)Glc1-ceramide; Gal(beta 1-4)[Fuc(alpha 1-3)]GlcNAc(beta 1-3)GlcNAc(beta 1-3)GalNAc(beta 1-4)Glc1-ceramide (Lewis X pentasaccharide structure); Gal(beta 1-4)[Fuc(alpha 1-3)]GlcNAc(beta 1-3)GlcNAc(pl 3)GalNAc(beta 1-4)Glc1-ceramide (Lewis X hexasaccharide structure); and, Fuc(alpha 1-3)Gal(beta 1-4) [Fuc(alpha 1-3)] GlcNAc(beta 1-3)GalNAc(beta 1- 1)Glc1-ceramide (pseudo-Lewis Y hexasaccharide structure), These structures belong to the characterized schisto-series of protostomial glycosphingolipids, The Lewis X and pseudo-Lewis Y glycolipids are stage-specifically expressed by the cercarial life-cycle stage, and not by the adult or egg.
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