Journal
JOURNAL OF BIOCHEMISTRY
Volume 128, Issue 3, Pages 399-405Publisher
JAPANESE BIOCHEMICAL SOC
DOI: 10.1093/oxfordjournals.jbchem.a022767
Keywords
apoptosis; DAD1; Mcl-1; N-linked glycosylation; tsBN7
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DAD1 is a mammalian, homologue of Saccharomyces cerevisiae Ost2p, a subunit of the oligosaccharyltransferase complex. Loss of its function induces apoptosis in hamster BHK21 cells. By means of a two-hybrid method involving DAD1 as bait, the C-terminal region of Mcl-1, one of the bcl-2 family, was isolated. Consistently, DAD1 binds well to Mcl-1 in COS cells when overexpressed, On deletion analysis, the C-terminal domain of Mcl-1 containing BH2 (bcl-2 homologous domain) was found to be essential for the interaction with DAD1, On the other hand, the C-terminal half of DAD1 was concluded to be essential for the interaction with Mcl-1, Surprisingly, a Delta C-DAD1 mutant lacking only 4 amino acid residues from the C-terminus did not complement the tsBN7 mutation, while it interacted well with Mcl-1, In contrast, Delta N-DAD1 lacking 20 amino acid residues from the N-terminus still exhibited the ability to complement the tsBN7 mutation. Thus, the C-terminus of DAD1 was suggested to play an important role in N-linked glycosylation and to complement the tsBN7 mutation. Mcl-1 may be required for the inhibition, of apoptotic cell death caused by a loss of DAD1.
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