Journal
GENES & DEVELOPMENT
Volume 14, Issue 17, Pages 2206-2215Publisher
COLD SPRING HARBOR LAB PRESS
DOI: 10.1101/gad.826100
Keywords
homologous recombination; DNA double-strand break repair; meiosis; DNA supercoiling
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Funding
- NIA NIH HHS [T32 AG000165] Funding Source: Medline
- NIEHS NIH HHS [ES07061, R01 ES007061] Funding Source: Medline
- NIGMS NIH HHS [GM57814, GM53738, R01 GM053738, R01 GM057814] Funding Source: Medline
- NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES [R01ES007061] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM057814, R01GM053738] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE ON AGING [T32AG000165] Funding Source: NIH RePORTER
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The first DNA joint formed in homologous recombination processes is a D-loop. Saccharomyces cerevisiae RDH54/TID1-encoded product, a Swi2/Snf2-like factor involved in recombination, is shown here to promote D-loop formation with Rad51 recombinase. Physical interaction between Rdh54 and Rad51 is functionally important because Rdh54 does not enhance the recombinase activity of the Escherichia coli RecA protein. Robust dsDNA-activated ATPase activity in Rdh54 generates unconstrained negative and positive supercoils in DNA. Efficient D-loop formation occurs with even topologically relaxed DNA, suggesting that via specific protein-protein interactions, the negative supercoils produced by Rdh54 are used by Rad51 for making DNA joints.
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