Journal
MOLECULAR AND CELLULAR BIOCHEMISTRY
Volume 206, Issue 1-2, Pages 169-175Publisher
KLUWER ACADEMIC PUBL
DOI: 10.1023/A:1007024726889
Keywords
testosterone; DHT; AR; ARE
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Funding
- NATIONAL CANCER INSTITUTE [R01CA055639, R29CA055639] Funding Source: NIH RePORTER
- NCI NIH HHS [CA55639] Funding Source: Medline
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There are two major physiological androgens, testosterone (T), and 5 alpha-dihydrotestosterone (DHT), which induce different responses in mammals. These androgens regulate the target gene transcription via binding to and activating the same androgen receptor (AR). The molecular mechanisms that differ between these two very close androgens through the same AR protein to target the distinct genomic responses remain unknown. Using yeast genetic selection, we identified two kinds of androgen response elements (ARE), which could respond differentially to T vs. DHT. These two AREs also show different T-vs. DHT-induced AR transactivation in mammalian Chinese hamster ovary (CHO) cells in terms of copy number and comparisons with the classic mouse mammary tumor virus ARE. Together, our results suggest that the selective ARE sequence may play an important role in the differential T- vs. DHT-induced AR transactivation.
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