Journal
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
Volume 23, Issue 13, Pages 2047-2056Publisher
MARCEL DEKKER INC
DOI: 10.1081/JLC-100100472
Keywords
-
Ask authors/readers for more resources
An improved high performance liquid chromatography (HPLC) method for the analysis of the main organic compounds in musts and wines is presented. A column packed with hydrogen sulfonated divinyl benzene-styrene copolymer and two detectors connected in series were used (UV at 210 nm and RI, respectively). The addition of acetonitrile (6%) to the mobile phase (0.045N H2SO4) allowed the simultaneous separation and quantification of several organic acids, glucose, fructose, glycerol, and ethanol. Direct injection and sample clean-up with a SAX cartridge was tested and compared. The SAX fractionation gave satisfactory results, however the direct injection of diluted wine (1:20) provided the best precision (CV less than or equal to 2.1%) and accuracy of analysis. Statistical analysis (paired t-test) disclosed significant differences only for glucose, fructose, alpha-ketoglutaric, and pyruvic acid. The role of column temperature for the analysis of vitamin C (ascorbic acid) was also investigated. Vitamin C underwent thermal degradation during analysis with column temperature greater than or equal to 30 degrees C.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available