Journal
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
Volume 23, Issue 12, Pages 1873-1885Publisher
MARCEL DEKKER INC
DOI: 10.1081/JLC-100100459
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An HPLC method for simultaneous identification and quantitation of tocopherols and carotenoids in red palm oil was developed. A C-30 column was used in a HPLC equipped with a photodiode detector and with methyl tertiary-butyl ether/ methanol/water as the mobile phase. Tocopherols and carotenoids were monitored at 295 nm and 450 nm, respectively. Gradient elution resulted in complete separation of five tocopherols such as alpha-, delta-, gamma-tocopherols and tocotrienols in palm oil in less than 10 minutes. Several (13) carotenoids were also detected and quantitated in red palm oil within 45 minutes.
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