Journal
ANALYTICAL LETTERS
Volume 33, Issue 5, Pages 861-867Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/00032710008543094
Keywords
hydrophobic membrane proteins; reversed phase chromatography; modified C18 reversed phase; penicillin binding proteins; cyanogen; time- course aggregation; protein complexes
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Hydrophobic proteins have proven to be difficult to separate chromatographically. The use of a C18 reversed phase column with embedded polar groups showed improved separation and recovery of the hydrophobic, membrane associated, penicillin-binding proteins of Bacillus subtilis and their covalently linked complexes. The covalently linked complexes were generated by a time course reaction of the non-covalently associated complexes in the intact cell with the paired group specific reagent, cyanogen (C2N2).
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