Journal
JOURNAL OF FOOD SCIENCE
Volume 65, Issue 1, Pages 96-100Publisher
INST FOOD TECHNOLOGISTS
DOI: 10.1111/j.1365-2621.2000.tb15962.x
Keywords
myofibril; protein extraction; polyphosphate; phase contrast microscopy
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Physical changes in chicken gastrocnemius myofibrils Incubated in 0.1 to 1.0 M NaCl solutions with or without 10 mM ortho-(P), pyro-(PP), tripoly-(TPP) or hexameta- (HMP) phosphate at pH 6.0 were examined by phase-contrast microscopy, electrophoresis, and solubility. PP and TPP performed similarly In promoting protein extraction, P had no apparent effect, and HMP exhibited an intermediate effect. PP, TPP, and HMP treatments markedly improved protein solubility in 0.3 and 0.4 M NaCl through the release of myosin, but the phosphate effect diminished in greater than or equal to 0.6 M NaCl. Overall, phosphates influenced the ultrastructure of myofibrils and extraction of their constituents in the order: PP approximate to TPP > HMP > P approximate to nonphosphate control.
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