Journal
NATURE CELL BIOLOGY
Volume 2, Issue 1, Pages 25-29Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/71345
Keywords
-
Categories
Funding
- Telethon [1226] Funding Source: Medline
Ask authors/readers for more resources
Cyclic AMP controls several signalling cascades within cells, and changes in the amounts of this second messenger have an essential role in many cellular events. Here we describe a new methodology for monitoring the fluctuations of cAMP in living cells, By tagging the cAMP effector protein kinase A with two suitable green fluorescent protein mutants, we have generated a probe in which the fluorescence resonance energy transfer between the two fluorescent moieties is dependent on the levels of cAMP, This new methodology opens the way to the elucidation of the biochemistry of cAMP in vivo.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available