Journal
PHYTOPATHOLOGY
Volume 90, Issue 9, Pages 973-976Publisher
AMER PHYTOPATHOLOGICAL SOC
DOI: 10.1094/PHYTO.2000.90.9.973
Keywords
host-selective toxin; molecular diagnostics
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Alternaria alternata apple pathotype (previously A. mali) causes Alternaria blotch on susceptible apple cultivars through the production of a host-specific toxin, AM-toxin. Identification of some Alternaria species, especially those that produce host-specific toxins, has been extremely difficult due to a high lever of variability which extends even to nonpathogenic isolates. We have recently cloned and characterized a gene (AMT) that plays a crucial role in AM-toxin biosynthesis and demonstrated that it is only present in isolates of A. alternata apple pathotype. Using primers designed for the AMT gene, we developed a polymerase chain-reaction-based method to specifically detect AM-toxin producing isolates of A. alternata apple pathotype.
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