Journal
ENZYME AND MICROBIAL TECHNOLOGY
Volume 27, Issue 6, Pages 356-361Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/S0141-0229(00)00232-5
Keywords
enzyme immobilization; amyloglucosidase; PS; PNaSS microsphere; hydrolysis of dextrin
Categories
Ask authors/readers for more resources
Nonporous polystyrene/poly(sodium styrene sulfonate) (PS/PNaSS) microspheres were used for immobilization of amyloglucosidase and the properties of immobilized enzyme was studied and compared with those of free enzyme. Sulfonated groups on the PS/PNaSS microspheres present a very simple, mild, and time-saving process for enzyme immobilization. Nonporous microspheres provide their surface for immobilization of enzyme and prevent the diffusion limitation problem in the pore. Despite the high concentration of bound enzyme the influence of immobilization on kinematic parameters, K-m and V-max, is relatively low compare to other porous supports. Simple and time-saving immobilization procedure as well as the effects of pH and temperature on immobilized enzyme also showed that the PS/PNaSS microspheres could be good support. (C) 2000 Elsevier Science Inc. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available