Journal
GENETICS AND MOLECULAR BIOLOGY
Volume 23, Issue 1, Pages 65-66Publisher
SOC BRASIL GENETICA
DOI: 10.1590/S1415-47572000000100011
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A modification of the CsCl gradient centrifugation method for DNA phage purification is presented. It avoids the enzymatic steps as well the need for a preliminary phage titration, a tedious process proposed in the majority of the protocols in use. The quality of the DNA obtained makes it amenable for additional manipulations like digestions, ligations, labelling, subcloning, etc.
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